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Open Access

ARTICLE

A Tritipyrum-Derived HVA22 Homolog Enhances Wheat Salt Tolerance

Tingting Yuan1,#, Mutong Li1,#, Zhishun Yu1, Jiangyun Huang1, Qingqin Zhang1, Suqin Zhang1,2,*, Guangdong Geng1,*
1 College of Agriculture, Guizhou University, Guiyang, China
2 Guizhou Subcenter of National Wheat Improvement Center, Guiyang, China
* Corresponding Author: Suqin Zhang. Email: email; Guangdong Geng. Email: email
# These authors contributed equally to this work.
(This article belongs to the Special Issue: Plant Responses to Abiotic Stress)

Phyton-International Journal of Experimental Botany https://doi.org/10.32604/phyton.2026.075984

Received 12 November 2025; Accepted 04 February 2026; Published online 14 February 2026

Abstract

HVA22 is a gene induced by abscisic acid (ABA) and abiotic stress. Previous transcriptome data of salt-tolerant Tritipyrum “Y1805” revealed that HVA22 was significantly upregulated under salt stress. Gene TtHVA22 was successfully amplified from “Y1805”, with an open reading frame of 468 bp and encoding a protein of 156 amino acids. Gene TtHVA22 was transformed into bread wheat “1718” via coleoptile method. The relative expression level of TtHVA22 in roots was remarkably higher than in stems and leaves under salt stress. During the seedling stage, the TtHVA22 overexpression (OE) line exhibited less leaf wilting under salt stress than wild-type (WT) plants. Under salt stress and recovery conditions, TtHVA22 OE significantly increased root length, plant height, fresh weight, and dry weight compared to WT plants. Additionally, the levels of ABA, soluble sugars, soluble proteins, proline, pyruvate, and photosynthetic pigments and peroxidase activity were significantly higher in the OE lines than in the WT plants; however, their malondialdehyde content and relative conductivity were opposite. Two years of field data demonstrated that stem diameter and grain yield per plant were significantly greater in the OE lines than the WT plants. Therefore, wheat salt tolerance was improved in the TtHVA22 OE lines by osmotic regulation, antioxidation, and chlorophyll stabilization.

Keywords

Tritipyrum; TtHVA22; gene cloning; expression pattern; salt tolerance

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