Standardizing immunohistochemistry methodology for evaluation of PD-1 and PDL-1 expression in upper tract urothelial carcinoma
Luca Campedel1,†, Thomas Seisen1,†, Justine Varinot2, Géraldine Cancel-Tassin2,3,#, Alain Ruffion4, Emilien Seizilles De Mazancourt4, Myriam Decaussin-Petrucci5, Grégoire Robert6, Nam-Son Vuong6, Magali Philipp7, Eva Compérat2,3,#, Morgan Rouprêt1,3,#, Olivier Cussenot2,3,#
Canadian Journal of Urology, Vol.28, No.3, pp. 10719-10724, 2021
Abstract Introduction: Controversy regarding the prognostic and/or predictive role of PD-1 and PD-L1 expression for upper tract urothelial carcinoma (UTUC) could partly be explained by inconsistencies in the immunohistochemistry (IHC) methodology. The objective is to standardize the methodology for routine evaluation of PD-1 and PD-L1 expression in UTUC patients.
Materials and methods: Twenty-two cases treated with radical nephroureterectomy between 1996 and 2015 at 11 French hospitals were randomly selected to compare different methodologies for evaluation of PD-1 and PD-L1 expression. IHC was carried out on whole tissue sections and 0.6 mm- or 2 mm-core tissue micro-arrays (TMAs) using… More >