Special Issue "Bacteriophage Biology and Biotechnology"

Submission Deadline: 20 January 2021 (closed)
Guest Editors
Dr. Xiangyu Fan, School of biological science and technology, University of Jinan.
Xiangyu Fan is a young scientist in the field of bacteriophage genome, phage-host interaction, specialising in mycobacteriophages. After completing his PHD's degree in 2014 at Southwest University, he joined the University of Jinan. He studied as a visiting scholar in the Institute for Environmental Genomics, University of Oklahoma in 2019. Now he is an associate professor at school of biological science and technology, University of Jinan.


Bacteriophages are the viruses of bacteria, which have contributed extensively to our understanding of modern biology. The phage-mediated bacterial growth inhibition represents an immense untapped source for novel antimicrobials. Thus, this special issue will try to gather the latest research findings in the field of phage, prophage and phage-host interactions.

Bacteriophage, Phage, Prophage, Temperate phage

Published Papers
  • The bacteriophage mu lysis system–A new mechanism of host lysis?
  • Abstract Bacteriophages are viruses that infect bacteria and can choose any one of the two alternative pathways for infection, i.e., lysis or lysogeny. Phage lysis is one of the conventional biological processes required to spread infection from one bacterium to another. Our analysis suggests that in the paradigm bacteriophage Mu, six proteins might be involved in host cell lysis. Mu has a broad host range, and Mu-like phages were found in both Gram-negative and Gram-positive bacteria. An analysis of the genomes of Mu and Mu-like phages could be useful in elucidating the lysis mechanism in this group of phages. A detailed… More
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  • Progresses of mycobacteriophage-based Mycobacterium tuberculosis detection
  • Abstract Tuberculosis (TB) remains a major cause of morbidity and mortality worldwide, particularly in developing countries. A rapid and efficient method for TB diagnosis is indispensable to check the trend of tuberculosis expansion. The emergence of drug-resistant bacteria has increased the challenge of rapid drug resistance tests. Due to its high specificity and sensitivity, bacteriophage-based diagnosis is intensively pursued. In this review, we mainly described mycobacteriophage-based diagnosis in TB detection, especially two prevalent approaches: fluorescent reporter phage and phage amplified biologically assay (PhaB). The rationale of reporter phage is that phage carrying fluorescent genes can infect host bacteria specifically. Phage amplified… More
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  • GP30 of the mycobacteriophage CASbig impairs mycobacterial adaptation during acidic stress and in macrophages
  • Abstract The rapid emergence of multidrug-resistant and extensively drug-resistant Tuberculosis retrieved intense interest in phage-based therapy. This old approach, which was abandoned in the west in the 1940s but is generating renewed interest, has stimulated fresh research on mycobacteriophages and their lytic efficiency against their hosts. GP30 is a novel protein of the mycobacteriophage CASbig with undiscovered function. In this study, we analyzed the role of CASbig gp30 in the host Mycobacterium smegmatis. Overexpression of gp30 in the host led to reduced growth in acidic medium and attenuated the intracellular survival rate of M. smegmatis inside the THP-1 macrophages, which may… More
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