Phytochemical Profiling and Pharmacological Evaluation of Astrantia major L. Herb Extracts with Systemic Anti-Inflammatory and Topical Hemostatic, Wound-Healing, and Antimicrobial Activities
Taras Koliadzhyn1, Andriy Grytsyk1, Nadiia Lehin1, Lubov Grytsyk2, Ain Raal3, Oleh Koshovyi3,4,*
1 Department of Pharmaceutical Management, Drug Technology and Pharmacognosy, Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
2 Department of Chemistry, Pharmaceutical Analysis and Postgraduate Education, Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
3 Institute of Pharmacy, Faculty of Medicine, University of Tartu, Tartu, Estonia
4 Departments of Clinical Pharmacy, Pharmacotherapy, Pharmacognosy, and Pharmaceutical Chemistry, Zaporizhzhia State Medical and Pharmaceutical University, Zaporizhzhia, Ukraine
* Corresponding Author: Oleh Koshovyi. Email: 
(This article belongs to the Special Issue: Medicinal Plants: Phytochemical and Pharmacological Research)
Phyton-International Journal of Experimental Botany https://doi.org/10.32604/phyton.2026.085064
Received 07 May 2026; Accepted 10 August 2026; Published online 12 August 2026
Abstract
Astrantia major L. (Apiaceae) is a non-official medicinal plant with a long history of use in the traditional medicine of many European countries; however, its phytochemical composition and pharmacological properties remain insufficiently studied. This study aimed to investigate the phenolic profile of
A. major herb extracts and evaluate their biological activities. A total of 17 phenolic compounds were identified, including hydroxycinnamic acids, flavonoids, catechins and tannin-related metabolites. Chlorogenic acid was the predominant hydroxycinnamic acid, while rutin was the major flavonoid. Among the tannin-related metabolites, gallocathechin predominated in the aqueous extract (AM0), whereas epigallocatechin was the major constituent in the hydroethanolic extract (AM70). The total phenolic content ranged from 14.42 ± 0.13% to 16.91 ± 0.09%. Pharmacological evaluation of aqueous and 70% hydroethanolic extracts demonstrated statistically significant anti-inflammatory activity compared to the pathological control group, reduced bleeding time, and accelerated wound healing. The extracts also exhibited selective antimicrobial activity against
Staphylococcus epidermidis and Escherichia coli. The selectivity pattern differed between the extracts. The observed biological activities are likely correlated with the phenolic profile of the plant.
A. major represents a promising source of biologically active compounds with hemostatic, wound-healing, and anti-inflammatory potential.
Keywords
Astrantia major; great masterwort; phenolic compounds; flavonoids; tannins; hydroxycinnamic acids; extracts; hemostatic; anti-inflammatory; antimicrobial; wound-healing activities
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